Fig. 6

LincSCRG1 promotes cell proliferation and migration of HCC via regulating the miR26a/SKP2 axis in vitro. Sh-NC, sh-lincSCRG1, sh-lincSCRG1 + ov-SKP2 and sh-lincSCRG1 + in-miR26a groups were established in SNU-387 and Hep-3B cell lines. a Cell viability was examined by MTT assays. b Oncogenic survival was assessed by colony formation assays. c Cell cycle proliferation was evaluated by flow cytometry. d Migration was determined by transwell assays. e Cell cycle-related proteins (CKD4/6 and cyclinD1) and EMT-related proteins (MMP-2/3/9, E-cadherin, N-cadherin and Vimentin) were examined by western blot analysis. In (a - d) ^*/#/&indicatesthe sh-lincSCRG1 vs. sh-NC group, the sh-lincSCRG1 + ov-SKP2 vs. sh-lincSCRG1 -group, and the sh-lincSCRG1 + in-miR26a vs. sh-lincSCRG1 group, respectively (n = 6). ^{* /#/&}, p < 0.05, ^**/##/&&, p < 0.01, ^***/###/&&&, p < 0.001