Fig. 2From: CircPVT1 promotes proliferation of lung squamous cell carcinoma by binding to miR-30d/eCircPVT1 promotes LUSC cell proliferation. a qRT-PCR analysis of circPVT1 and PVT1 RNA expression in H520 cells after treatment with circPVT1 expression vector, mock, si-cPVT1 or NC; b Assessment of proliferation in H520 and H2170 cells transfected with circPVT1 expression vector, mock, si-cPVT1 or NC by CCK-8 assay; c and d Assessment of DNA synthesis using an EdU assay in H520 and H2170 cells transfected with circPVT1 expression vector, mock, si-cPVT1 or NC. Micrographs represent at least 3 experiments. Scale bar = 200 μm. e and f. Colony formation assay in H520 and H2170 cells transfected with circPVT1 expression vector, mock, si-cPVT1, or NC. Data were showed as mean ± SD, *P < 0.05; **P < 0.01; ***P < 0.001Back to article page