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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: CircPVT1 promotes proliferation of lung squamous cell carcinoma by binding to miR-30d/e

Fig. 2

CircPVT1 promotes LUSC cell proliferation. a qRT-PCR analysis of circPVT1 and PVT1 RNA expression in H520 cells after treatment with circPVT1 expression vector, mock, si-cPVT1 or NC; b Assessment of proliferation in H520 and H2170 cells transfected with circPVT1 expression vector, mock, si-cPVT1 or NC by CCK-8 assay; c and d Assessment of DNA synthesis using an EdU assay in H520 and H2170 cells transfected with circPVT1 expression vector, mock, si-cPVT1 or NC. Micrographs represent at least 3 experiments. Scale bar = 200 μm. e and f. Colony formation assay in H520 and H2170 cells transfected with circPVT1 expression vector, mock, si-cPVT1, or NC. Data were showed as mean ± SD, *P < 0.05; **P < 0.01; ***P < 0.001

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