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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: CircPVT1 promotes proliferation of lung squamous cell carcinoma by binding to miR-30d/e

Fig. 3

CircPVT1 is actively induced by HuR. a Silver staining of biotinylated circPVT1-associated proteins; b Western blotting of HuR from circPVT1 pull-down assays; c RIP confirmed the relationship between circPVT1 and HuR in H520 cells. GAPDH mRNA was used as a non-HuR target control; d The mRNA and protein level of HuR were significantly reduced by a HuR-siRNA as determined by qRT-PCR (below) and western blot (up); e CircPVT1was markedly reduced by HuR-siRNA as established by qRT-PCR; f The mRNA and protein level of HuR was markedly elevated by a vector-HuR as determined by qRT-PCR (below) and western blot (up); g CircPVT1was markedly upregulated by vector-HuR as determined by qRT-PCR; h The mRNA and protein level of HuR were not changed by circPVT1siRNA or circPVT1 expression vector determined by qRT-PCR (left) and western blot (right). Data are shown as mean ± SD, **P < 0.01;***P < 0.001

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