Fig. 4

CirPVT1 binds to miR-30d and miR-30e. a The cluster heat maps displayed the miRNAs binding to circPVT1; b Screened miRNA that possibly binds to circPVT1; c A luciferase reporter assay was used to detect the luciferase activity of LUC-cPVT1 in H520 cells transfected with miRNA mimics to identify miRNAs that bind to the circPVT1 sequence; d A schematic drawing shows the putative binding sites of miR-30d and miR-30e for circPVT1; e A luciferase reporter assay was used to detect the luciferase activity of LUC-cPVT1 or the LUC-cPVT1-mutant in H520 cells co-transfected with miRNA 30d mimics (down) and miRNA 30e mimics (up); f The relative level of miR-30d and miR-30e was measured by RT-qPCR in the H520 cells transfected with circPVT1 expression vector, mock, si-cPVT1 or NC; g Assessment of proliferation in H520 cells after 72 h transfected with circPVT1 siRNA or miR-30d inhibitors or miR-30d inhibitors by CCK-8 assay. Data are shown as mean ± SD, **P < 0.01;***P < 0.001