Fig. 5

CCNF is a direct target of miR-30d and miR-30e in LUSC cells. a The cluster heat maps displayed the upregulated and downregulated mRNAs. Each column indicates a sample while each row indicates an individual mRNA. The red and blue strips represent high and low expression, respectively; b Screened mRNA that possibly binds to miR-30d and miR-30e simultaneously; c The mRNA level of CCNF and DEPDC1B were significantly reduced by a circPVT1siRNA as determined by qRT-PCR; d The mRNA level of CCNF were markedly increased by miR-30d inhibitors and miR-30d inhibitors as determined by qRT-PCR; e A schematic drawing shows the putative binding sites of miR-30d and miR-30e with respect to CCNF; f A luciferase reporter assay was used to detect the luciferase activity of LUC-miR-30d or the LUC-miR-30d-mutant in H520 cells co-transfected with CCNF; g A luciferase reporter assay was used to detect the luciferase activity of LUC-miR-30e or the LUC-miR-30e-mutant in H520 cells co-transfected with CCNF; h The correlation between CCNF and miR-30d in LUSC was evaluated in TCGA database; i The correlation between CCNF and miR-30e in LUSC was evaluated in TCGA database. j Immunoblot analysis of CCNF protein expression after co-transfection with circPVT1 siRNA or miR-30d/30e inhibitors. Data are shown as mean ± SD, **P < 0.01;, ***P < 0.001