Fig. 1

LncRNA ARST was significantly downregulated in the gliomas. A The differential expression clusters of lncRNAs in the glioma specimens and paracancerous tissues were shown. The suffix E-Signal represented edge tissues, the suffix T-Signal represented tumor tissues and the suffix N-Signal represented normal tissues. The red color indicated the upregulated lncRNAs and the green color indicated the downregulated lncRNAs (left). The expression levels of ARST in the different tissues were displayed (right). B LncRNA location and PhyloCSF value analysis were shown by UCSC genome browser with PhyloCSF data hub. The differential expression levels of ARST in the clinical glioma specimens classified into low grade glioma (LGG), glioblastoma multiforme (GBM) tissues C or four World Health Organization (WHO) grades D were examined using qRT-PCR. E The expressions of ARST in the two glioma cell lines were tested by qRT-PCR compared to normal astrocytes (NHA). Each bar represents mean ± s.d. from three independent experiments. F Fluorescence in situ hybridization (FISH) assay was performed to detect the location of ARST in the U87MG cells. Scale bar = 5 μm. Human 18S was used as a cytoplasm internal control and human U6 was used as a nucleus internal control (left). Proportions of ARST and the internal controls were determined in the cytoplasm and nucleus of the cells (right). Data were presented as mean ± s.d. from three independent experiments (****P < 0.001)