Fig. 2

ARST inhibited the malignant phenotypes of the glioma cells. A The efficiencies of ARST overexpression in the U87MG and U251 cells were tested by qRT-PCR. B The growth curves of the transfected glioma cells were shown using CCK-8 assay. C The EdU staining assay was performed to determine the proliferation of the transfected U87MG and U251 cells. Scale bar = 100 μm. D Apoptosis of the transfected cells were analyzed by staining of Annexin V and PI followed by flow cytometry assay. E Migration and invasion of the transfected cells were determined by transwell assay. Scale bar = 100 μm. F Wound-healing assay was performed. Scale bar = 200 μm. G Representative images and total flux of the C57 mice 7 days and 14 days after intracranial implantation of the GL261 cells transfected with luciferase tagged ARST or control plasmids by IVIS spectrum. H Surviving curves of the C57 mice with xenografted tumors intracranially was recorded for 15 days. All data were presented as mean ± s.d. from three independent experiments (*P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.001)