Fig. 5

ARST decreased the interactions of ALDOA and cofilin with F-actin, respectively. A, B Co-immunoprecipitation assay was performed to detect the binding capacity of F-actin and ALDOA or cofilin in the U87MG cells transfected with ARST or negative control vector. C Co-staining of cofilin and phalloidin labelled F-actin cytoskeleton was performed in the transfected cells. Images were taken under confocal microscope. Scale bar = 2 μm (left) / 0.45 μm (right). D Transcripts per million (TPM) of cofilin in different cancers based on the TCGA database. E Overall and F disease free survivals of the glioma patients with relative low or high level of cofilin expressions were assessed in the GEPIA database (cut-off value is 50%)