Fig. 6

ARST interacted with the binding sites of ALDOA and F-actin. A, B The catRAPID database was referenced to predict the binding domains of ARST and ALDOA. C Truncated ARST was demonstrated by the diagrammatic sketch. The binding domain predicted by catRAPID was highlighted in yellow. D RNA pulldown followed by western blot was performed to detect the binding capacity of truncated ALDOA with ARST. E The mutation sites on ARST, highlighted by red arrows, were demonstrated by diagrammatic sketch. F RNA pulldown followed by western blot was performed to detect the interactive capacities of different mutant ALDOA with ARST. G Co-immunoprecipitation assay was performed to detect the binding capacity of F-actin and cofilin in the presence of ALDOA mutants. All results were presented as mean ± s.d. from three independent experiments (****P < 0.001)