Fig. 2

TRIM37 is critical for the migration, invasion and EMT process of RCC cells. A, Expression intensity of TRIM37 in RCC cell lines (Caki-1, Caki-2, 786-O, ACHN) was higher than normal kidney lines (HK-2). B, Validation of knocking down or overexpressing TRIM37 cell models (Caki-1, 786-O). C-E, Knockdown of TRIM37 attenuated cell migration and Matrigel invasion ability, overexpression of TRIM37 enhanced cell migration and Matrigel invasion ability. F, Overexpression of TRIM37 in RCC cells gradually exhibited a spindle-shaped and more elongated morphology. G, Knockdown of TRIM37 inhibited the expression of mesenchymal markers including N-cadherin, Vimentin and increased the expression of epithelial marker Claudin-1 in Caki-1 and 786-O lines. Overexpression of TRIM37 displayed adverse trends. H, Upregulating TRIM37 promoted 3D soft agar colony formative capability. I, Upregulating TRIM37 promoted 3D tumor spheroid invasive potential