Skip to main content

Table 1 ddPCR primers and probes information for this study

From: Circulating tumor DNA tracking through driver mutations as a liquid biopsy-based biomarker for uveal melanoma

 

Oligos

Annealing Temperature

GNA11-F

CTTTCAGGATGGTGGATGT

 

GNA11-R

ACATGATGGATGTCACGTTCT

58 °C

GNA11_A_Allele

5HEX/AC + CGC + TGG + CC/3IABkFQ

 

GNA11_C_Allele

56-FAM/AC + CGC + GGGCC/3IABkFQ

 

GNA11_T_Allele

56-FAM/AC + CGC + AGG + CC/3IABkFQ

 

GNAQ-F

CTTGCAGAATGGTCGATGTAG

 

GNAQ-R

GCGCTACTAGAAACATGATAGAG

60 °C

GNAQ_A_Allele

5HEX/CCT + T + T + G + GCCC/3IABkFQ

 

GNAQ_Q209L_T_Allele

56-FAM/CCT + T + A + G + GCCC/3IABkFQ

 

GNAQ_Q209P_C_Allele

56-FAM/ACCT+T + G + GGCC/3IABkFQ

 

PLCb4-F

TAACAAACGGCAAATGAGTCG

 

PLCb4-R

CAGCCAGCGTTCCAGAAA

55 °C

PLCB4_D630Y_G_allele

5HEX/CGA + GT + C + G + ATT + CC/3IABkFQ

 

PLCB4_D630Y_T_allele

56-FAM/CGA + GT + C + T + AT+TC + CA/3IABkFQ

 

CYSLTR2-F

CCTTGTATGTCAACATGTACAGC

 

CYSLTR2-R

GTGAACCATTGCCAGGAAAC

55 °C

CYSLTR2_T_allele

5HEX/TTC + C + T + GA + CCGT/3IABkFQ

 

CYSLTR2_A_allele

56-FAM/TTC + C + A + GA + CCGT/3IABkFQ

Â