Integrated multi-omics analyses on patient-derived CRC organoids highlight altered molecular pathways in colorectal cancer progression involving PTEN

Table 4 Genetic alterations assessed through targeted NGS profiling in CRC primary tissues and relative PDCOs

Sample ID	Gene ID	Protein Change	VAF (%)	COSMIC Classification
P12T primary tissue	PIK3CA	P449T	16	Pathogenic
	APC	R876X	34	Pathogenic
	APC	E1554X	28	Pathogenic
	PTEN	F273X	7	Unknown
	KRAS	G12V	26	Pathogenic
P12T -W early	PIK3CA	P449T	38	Pathogenic
	APC	R876X	50	Pathogenic
	APC	E1554X	50	Pathogenic
	PTEN	F273X	58	Unknown
	PTEN	R335X	20	Pathogenic
	KRAS	G12V	50	Pathogenic
P14T primary tissue	PIK3CA	E545G	12	Pathogenic
	APC	E1379X	22	Pathogenic
	KRAS	G13C	12	Pathogenic
	TP53	R196X	18	Pathogenic
P14T -W early	PIK3CA	E545G	50	Pathogenic
	APC	E1379X	100	Pathogenic
	KRAS	G13C	50	Pathogenic
	TP53	R196X	100	Pathogenic

For every variant, we indicate the variant allele fraction (VAF) and the putative pathogenicity

ISSN: 1756-9966