Fig. 5

HOMER3 enhances T24 cells proliferation and invasion in normoxia and, particularly, under oxygen shortage and glucose deprivation. T24 wild type cells (T24WT), CRISPR-Cas9 control cells (CTRL), T24_HOMER_KI (with HOMER3 elevation) and KD1–2/KO1 (with HOMER3 downregulation/abrogation) models, all showed decreased proliferation and increased invasion in hypoxia plus glucose deprivation in comparison to normoxia. Notably, T24_HOMER3_KI models were significantly more proliferative and invasive in comparison to wild type and control cells in normoxia and, particularly, under hypoxia and nutrient shortage. The T24_ HOMER3_KD/KO showed the opposite behavior. The results are the average of at least three independent replicates. *p < 0.05; **p < 0.01; ***p < 0.001; **** p < 0.0001 (two-way ANOVA with interaction followed by Tukey post hoc)