Fig. 6

Target genes of OICR-9429 are identified in bladder cancer. a. Volcano plot showing the differentially expressed genes in RNA sequencing in T24 and UM-UC-3 cells treated with DMSO or 140 μM OICR-9429 for 48 h. b. A heatmap representing both downregulated mRNA expression levels in T24 and UM-UC-3 cells treated with OICR-9429. c. The differentially expressed genes in the RNA sequencing were verified in T24 and UM-UC-3 cells by qRT-PCR. d. The expression of OICR-9429 target genes in T24 and UM-UC-3 cells was detected by Western blots. GAPDH and histone H3 were used as internal controls. e. ChIP-qPCR analysis of H3K4 tri-methylation and Pol-II status in the promoters of target genes after treatment with DMSO or 140 μM OICR-9429 for 48 h in T24 and UM-UC-3 cells. Values were normalized to the DMSO groups. *p < 0.05 and **p < 0.01