Fig. 7
WDR5 positively correlates with PD-L1, and OICR-9429 suppresses immune evasion by blocking PD-L1 activation. a. Pearson correlation between the expression of WDR5 and PD-L1 in the TCGA cohort. b. c. Relative mRNA (b) and protein (c) expression of PD-L1 in two BCa cell lines treated with DMSO, IFN-γ, IFN-γ + IC50 OICR-9429, and IFN-γ + 2 IC50 OICR-9429. GAPDH was used as the internal control. d. Flow cytometry verified that OICR-9429 reduced PD-L1 expression induced by IFN-γ treatment in two BCa cell lines. e. ChIP-qPCR analysis of H3K4 tri-methylation and Pol-II status in the PD-L1 promoter after treatment with IFN-γ or IFN-γ + 2 IC50 OICR-9429 for 48 h in T24 and UM-UC-3 cells. Values were normalized to the IFN-γ group. f. A schematic model of the mechanism underlying the role of OICR-9429 in BCa proliferation, chemosensitivity, metastasis and immune evasion. *p < 0.05 and **p < 0.01