Fig. 1

LINC00511 was highly expressed and related to vesicle trafficking. a LINC00511 expression in HCC from TCGA (T = 374 cases, N = 50 cases). b Violin plot shows the relative LINC00511 expression in different stages of HCC in a TCGA HCC cohort (n = 374). c Kaplan-Meier survival curves generated from TACG cohorts (n = 374). d LINC00511 expression in HCC patient tumor (T) tissue with paired adjacent non-tumor (NT) samples (n = 30). e Enrichment plot showing enrichment of vesicle trafficking-related genes in the LINC00511 high expression group. f KEGG pathway analysis that the high expression of LINC00511 group was related to vesicle-mediated transport and actin cytoskeleton organization. g, h Immunofluorescence of in HCC and adjacent tissues stained with CD63 (green) and RAB7 (red), respectively. Insets show higher magnifications of boxed areas. Arrowheads mark CD63 and RAB7 positive spots. Scale bar, 20um. i, j Electron microscopy images showing MVBs in HCC and adjacent tissues. White dotted line indicated MVBs. Scale bar, 500 nm. Quantification of MVBs numbers in HCC and adjacent tissues. Data are mean ± SD from three independent experiments, t-test *, P < 0.05, **, P < 0.01