Fig. 2

LINC00511 promotes exosome secretion in HCC cells. a Exosomes from Huh7 cells assessed by transmission electron microscopy. Scale bar, 200 nm. b Exosomes from Huh7 and Hep3B cells transfected with Ctrl or LINC00511 assessed by immunoblotting. Exosomes were analyzed for classical exosome markers CD63, CD81, and TSG101. c-e Quantification of CD63, CD81 and TSG101 levels in exosomes isolated from for each condition in (B). The relative intensities were normalized to those from Huh7 and Hep3B cells transfected with Ctrl. f The size distribution of exosomes derived from Huh7 cells assessed by nanoparticle tracking analysis (NTA). g NTA analysis of the effect of LINC00511 on exosome release in Huh7 and Hep3B cells. h LINC00511(green) expresses in the cytoplasm as detected by in situ hybridization FISH in Huh7 cells. Scale bar, 100 μm. Data are mean ± SD from three independent experiments, t-test *, P < 0.05, **, P < 0.01