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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: LINC00511 drives invasive behavior in hepatocellular carcinoma by regulating exosome secretion and invadopodia formation

Fig. 7

LINC00511 promotes the invasion of HCC cells via a reciprocal relationship between invadopodia and exosomes. a, b Huh7 and Hep3B cells co-transfected with LINC00511 and si-VAMP7 or co-culture with Ctrl-Exo and LINC00511-Exo were seeded on Matrigel-coated cell inserts and allowed to invade for 24 h, and then invasive cells were counted. Scale bar, 200 um. c, d Representative images and quantification of the invasive ability of Huh7 and Hep3B cells co-transfected with LINC00511 and si-VAMP7 or co-culture with Ctrl-Exo and LINC00511-Exo. After 48 h of invasion, serial optical sections (10 μm interval) were acquired. Red arrow indicates the direction of invasive movement. Scale bar, 100 μm. e-g Representative images of the tumors recovered at the end of the experiment. Tumor volume was calculated at the indicated time points and tumor weight was measured in Vector and LINC00511-OE groups at the end of the experiment. h Immunofluorescence of tissues from Vector and LINC00511-OE groups stained with invadopodia markers cortactin (green) and actin (Phalloidin-iFluor 594). Scale bars, 100 μm. Data are mean ± SD from three independent experiments, t-test *, P < 0.05, **, P < 0.01

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