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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: RNA-binding protein IMP3 is a novel regulator of MEK1/ERK signaling pathway in the progression of colorectal Cancer through the stabilization of MEKK1 mRNA

Fig. 3

IMP3 promoted CRC cell proliferation, migration and invasion in vitro. (A) The baseline RNA level of IMP3 in eight CRC cell lines detected by RT-qPCR. (B) The baseline protein level of IMP3 in eight CRC cell lines detected by western blot. (C) Efficiencies of IMP3 knockdown was detected in indicated cells transfected with siRNAs by RT-qPCR (* p < 0.05). (D) Efficiencies of IMP3 overexpression was detected in indicated cells transfected with plasmids by RT-qPCR (* p < 0.05). (E) Efficiencies of IMP3 knockdown or overexpression was detected in indicated cells transfected with siRNAs or plasmids by western blot. (F) CCK8 assays showed that knockdown of IMP3 reduced cell viabilty in RKO and HCT116 cell line (* p < 0.05), and upregulation of IMP3 increased cell viability in LOVO and SW480 cell line (* p < 0.05). (G) Colony formation assays for indicated cells after transfection with siRNAs or plasmids (* p < 0.05). (H) Representative images (40×) of wound healing assays for indicated cells (* p < 0.05). (I) Representative images (200×) of transwell invasion assay for indicated cells (* p < 0.05)

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Journal of Experimental & Clinical Cancer Research

ISSN: 1756-9966

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