Fig. 3

Activation of mitochondrial pathway and caspase 3 are required for cleavage of GSDME and pyroptosis induced by TPL. a The protein levels of mitochondrial death pathway were determined by western blot. b sub-cellular distributions of BAX, BAD and cytochrome c proteins were determined by subcellular fraction and western blot assays. c The mRNA and protein levels of BAX, BAD in siRNAs transfected cells were determined by qPCR or western blot. d Cleavage of caspase 3 and GSDME in BAX/BAD-depleted cells were measured by western blot. e Morphological features showed less cell swelling and rupture induced by TPL when caspase activity was blocked by z-VAD. f Effects of z-VAD on cleavage of GSDME was measured by western blot. g The mRNA and protein levels of caspase 3 in siRNAs transfected cells were determined by qPCR or western blot. h Cleavage of GSDME in caspase 3 silenced cells were determined by western blot