Fig. 4

TPL suppresses mitochondrial HK-II and glycolysis in head and neck cancer cells. a GSEA analysis indicated TPL induced transcriptomic alterations were associated with c-myc targets and glycolysis. b qPCR assay showed that TPL suppressed c-myc, HK-II mRNA levels in head and neck cancer cells in a dose-dependent manner. c The protein levels of HK-II were measured by western blot. d distribution of HK-II upon TPL treatment was determined by subcellular fraction and western blot. e co-localization of HK-II with mitochondria was assessed by immunofluorescence assay. f glycolytic rates were assessed by glucose consumptions, lactate productions and cellular ATP contents assays. *P < 0.05, **P < 0.01, ***P < 0.001