Fig. 5

Mitochondrial HK-II prevents TPL induced pyroptosis. a The mRNA and protein levels of HK-II in shRNAs or cDNA expressing lentivirus infected cells were assessed by qPCR or western blot. b Phase contrast images showed morphological features HK-II silenced or HK-II overexpressed cells upon TPL treatment. c Subcellular fraction assays suggested that silencing HK-II promoted mitochondrial translocation of BAX, BAD. d Silencing HK-II facilitated activation of caspase 3 and cleavage of GSDME upon TPL treatment, whereas overexpression of exogenous HK-II exerted opposite effects. e ELISA assays showed silencing HK-II promoted release of IL-1β into extracellular space, whereas overexpression of exogenous HK-II reduced the release of IL-1β upon TPL treatment. f LDH1 activity assays. g cell survival of HK-II depleted or HK-II overexpressed cells were assessed by colony formation assays. Values are shown as mean ± SD from triplicate experiments. *P < 0.05, **P < 0.01, ***P < 0.001