Fig. 6From: A HIF1α-GPD1 feedforward loop inhibits the progression of renal clear cell carcinoma via mitochondrial function and lipid metabolismOverexpression of GPD1 inhibited lipid metabolism in ccRCC cells. (A) KEGG analysis and gene ontology (GO) analysis of the differentially expressed genes (|log2FC| > 1, FDR-adjusted p < 0.05) in 786O-NC and 786O-GPD1 cells. (B) GSEA revealed that sterol biosynthetic gene sets, fatty acyl-CoA metabolism gene sets, and sterol metabolism gene sets were less enriched in 786O-GPD1 cells (FDR < 25% and p < 0.05). (C) Western blotting of indicated proteins in Caki1-NC, Caki1-GPD1, 769P-NC and 769P-GPD1 cells. (D) Images and quantifications (at 560 nm wavelength) of Oil Red O staining in Caki1-NC, Caki1-GPD1, 769P-NC, and 769P-GPD1 cells. (E) Quantitation of free fatty acids in Caki1-NC, Caki1-GPD1, 769P-NC, and 769P-GPD1 cells. Data are shown as the mean + SD (n = 3). Statistical analysis was performed using an unpaired Student’s t test with a two-tailed distribution (* p < 0.05, **p < 0.01). Abbreviations: GPD1, glycerol-3-phosphate dehydrogenase 1; GSEA, Gene Set Enrichment Analysis; KEGG, Kyoto encyclopedia of genes and genomes; ccRCC, clear cell renal cell carcinoma; CCK-8, cell counting kitBack to article page