Fig. 4

CD151 and integrin α3β1 were highcorrelated. a-b Human Soluble Receptor Antibody Array analysis of stable A549 cells in which CD151 either silenced or overexpressed. The list of up-regulated proteins in CD151-overexpressed A549 cells and the down-regulated proteins in CD151-silenced A549 cells are also shown. c The integrins were verified in A549 and H1299 stable cells using western blot. d Co-immunoprecipitation of CD151 and integrin and p-EGFR are shown. Protein were immunoprecipitated and detected from lysates of A549 and H1299 cells using a specific monoclonal antibody. e Data from CPTA database showed the correlation of CD151 and integrin. f Immunofluorescence staining of CD151 and integrin α3 co-expression in CD151-knockdown cells compared to control cells (Scale bar: 5 μm). *P < 0.05; **P < 0.01; ***P < 0.001