Fig. 6

Abnormal expression of CD151 disrupts EGF signalling. a Western blot assay of CD151, p-EGFR, p-ErbB2, p-FAK, p-Src, p-AKT, p-Erk, Cyclin D1, MMP2 and MMP9 expression in stable CD151 knockdown or overexpressed cells when compared to control cells. β-actin was used as a loading control. b Immunofluorescence staining of CD151 and integrin p-EGFR co-expression in CD151-knockdown cells compared to control cells (Scale bar: 5 μm). c Control and CD151-knockdown cells were treated with EGF (50ng/ml) for 1 h, and the expression levels of various proteins were then measured by western blot analysis. β-actin was used as the internal control. Bars represent mean ± SD from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001