Fig. 2

Expression of oncogenic and CSC markers in cell lines and donor PC cell samples. A. Expression of several notable oncogenes—EGFR, HER2, YAP1, MYC, and SOX9—was assessed by Western blotting in the three new cell lines, normal gastric epithelial cell line GES-1, and commercial GAC cell lines Snu-1 and MKN45. B. Expression of several notable CSC markers (YAP1, EpCAM, ALDH1, and SOX9) in the three new cell lines was determined by immunofluorescence staining as described in Materials and Methods. C. Expression of CD44, CD133, ALDH1 and YAP1 was evaluated by CyTOF in the three cell lines as described in Materials&Methods. D. ALDH1+ cells were detected using ALDEFLUOR detection kit in three cell lines. All three cell lines had varying proportions of ALDH1+ cells with the highest proportion in GA0518 compared with GA0804 and GA0825