Fig. 5

Molecular and gene expression features were preserved in PDXs compared with donor PC cells. A & B. Whole-exome sequencing was performed in GA0518- and GA0825-derived PDXs and on donor PC cells directly from patients according to the Materials and Methods. Mutations in GA0518- and GA0825-derived PDXs and donor PC cells were analyzed by a bioinformatician (S.Z.) (A). Copy-number variation status of GA0518- and GA0825-derived PDXs compared with the donor PC cells was analyzed as well. Common copy-number variations between PDXs and donor PC cells are shown (B). C. RNA-seq was performed in the GA0518 cell line, the corresponding donor PC cells (IP-013), and PDX tumors from the GA0518 and GA0825 cell lines, and global gene expression profiles were compared between the GA0518 cell line and donor PC cells (left), between the GA0518-derived PDX and donor PC cells (middle), and between the GA0825-derived PDX and donor PC cells (IP-116). Significantly positive correlation in global gene expression was observed for each comparison (p < 0.0001). D-F. Phenotypes of PDXs and donor PC cells were examined by hematoxylin and eosin (HE) and immunofluorescence staining for the CSC markers YAP1, EpCAM, ALDH1, and SOX9. All these markers were preserved in PDXs, recapitulating expression in the corresponding donor PC cells