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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Patient-derived cell lines and orthotopic mouse model of peritoneal carcinomatosis recapitulate molecular and phenotypic features of human gastric adenocarcinoma

Fig. 6

Improved orthotopic PDO model of PC. A. Illustration of the procedure of improved PDO model. B. In two representative mice, GA0518 cells generated primary stomach tumors and PC, imaged by BLI over a 3-week period. C. Representative PC and malignant ascites from a GA0518 cell–injected PDO model are shown. D. Summary of the tumorigenicity and PC metastases of the three new cell lines and two commercial GAC cell lines in our improved PDO models. E. RNA-seq was performed in PC cells and matching stomach tumors from the same GA0518 PDO mice as described in Materials&Methods. Significant gene changes either up or down were analyzed by bioinformatician (S.Z). F. Gene Ontology analysis was performed to identify the key genes in PC metastases involved in many important pathways, including hallmark of hypoxia, glycolysis, Wnt/β-catenin signaling, and TP53 pathways

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