Fig. 6

APX3330 suppressed cervical cancer metastasis in vivo. a APX3330 treatment reduced the incidence of lymph node metastasis in lymph node metastasis models. Lymph node metastatic models were generated with GFP-expressing HeLa cells (HeLa-GFP). Fluorescence image: Tumor metastasis was monitored by fluorescence imaging at day 28 after cell injection; Anatomic image: Arrow indicates metastatic tumors; LN H&E: Hematoxylin and eosin (H&E) staining of lymph nodes (LNs); Tumor cells: Green color indicates GFP-expressing HeLa cells in lymph nodes. Blue, DAPI. Data were tested by the chi square test. b APX3330 treatment inhibited the incidence of liver metastasis in cervical cancer lymph node metastasis models. MRI image: Liver metastasis of tumor was detected by MRI at day 28 after cell injection; Anatomic image: The circle and arrow indicates metastatic tumor nodules on the surface of the liver; H&E: H&E staining of liver tissues; The circle and arrow indicates metastatic lesions in the liver. Tumor cells: Green indicates GFP-expressing HeLa cells in the liver. Blue, DAPI. Data were tested by the chi square test. c APX3330 treatment reduced cervical cancer abdominal cavity metastasis. Xenograft models were generated by intraperitoneal injection of HeLa-GFP cells into mice. Fluorescence image: Tumor metastasis was monitored by fluorescence imaging on day 21 after cell injection. Anatomic image: Arrow indicates metastatic tumors. H&E: Hematoxylin and eosin (H&E) staining shows metastatic lesions in the abdominal cavity. The circle and arrows indicate metastatic tumors. Data are presented as the mean ± SD and analyzed by unpaired t-tests. *, P < 0.05; **, P < 0.01; ***, P < 0.001. d APX3330 treatment inhibited cervical cancer cell lung metastasis. Lung metastatic models were generated by tail vein injection of HeLa-GFP cells into mice. Fluorescence image: Tumor metastasis was monitored by fluorescence imaging on day 28 after cell injection. Anatomic image: Arrow indicates metastatic tumor nodules on the lung surface. H&E: Hematoxylin and eosin (H&E) staining shows metastatic lesions in the lung. Arrows indicate the metastatic tumors. PBS, mice were treated with PBS by I.P. injection. **, P < 0.01; ***, P < 0.001