Fig. 2

HCK is required for the proliferation of human AML cell lines. (a) Immunoblotting analysis of HCK expression in different leukaemia cell lines, including KG-1α, HEL, K562, NB4, THP-1, U937, and MV4-11. (b) Cells were transfected with shRNAs targeting HCK, followed by immunoblotting for HCK. (c) The numbers of THP-1, U937, and MV4-11 cells were counted at the indicated days after infection with shRNA targeting HCK or SCR. (d) Representative images of colonies formed by HCK-knockdown THP-1 cells after 14 days. (e) Quantification of colony numbers in d. (f) Representative flow cytometric analysis of the cell cycle distribution in THP-1 cells transfected with shRNA targeting HCK or SCR was determined using a BrdU incorporation assay. (g)Representative flow cytometric analysis of apoptosis in THP-1 cells transfected with shRNA targeting HCK or SCR. (h)Representative flow cytometric analysis of the differentiation in THP-1 cells transfected with shRNA targeting HCK or SCR