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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: HCK maintains the self-renewal of leukaemia stem cells via CDK6 in AML

Fig. 6

HCK may regulate CDK6 expression through the ERK1/2-c-Myc axis. (a) GSEA plots evaluating changes in c-Myc dependent gene signatures upon HCK deletion. FDR q-val, false discovery rate q-value; NES, normalized enrichment score. (b) Immunoblotting for HCK, CDK4, CDK6, c-Myc, ERK1/2, and p-ERK1/2 in LSCs, THP-1 and U937 cells. (c) Transfection of cells with shRNAs targeting c-Myc or CDK6, followed by immunoblotting for c-Myc and CDK6 and quantification of c-Myc or CDK6 protein level by densitometric analysis. (d) The numbers of THP-1 and U937 cells were counted on the indicated days after infection with shRNA targeting c-Myc or with scrambled shRNA. (e) The numbers of THP-1 and U937 cells were counted on the indicated days after infection with shRNA targeting c-Myc or with shRNA targeting CDK6. (f) Immunoblotting for CDK6 and c-Myc after treatment with an ERK1/2 inhibitor in U937 or THP-1, and quantification of c-Myc or CDK6 protein level by densitometric analysis. (g) Schematic representation of the HCK—ERK1/2—c-Myc—CDK6 protein network in AML

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