Skip to main content
Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: USP1-dependent RPS16 protein stability drives growth and metastasis of human hepatocellular carcinoma cells

Fig. 1

USP1 interacts with and regulates RPS16. a Endogenous USP1 was immunoprecipitated from HepG2 cells. The USP1-interacting proteins were separated by SDS-PAGE and were presented by silver staining assay. b Biological process analysis of the USP1-interacting proteins was performed. The numbers of regulated genes in pathway categories are shown. c Representative USP1-interacting proteins are shown in the table. d USP1 was immunoprecipitated from HepG2 cells and immunoblotted to RPS4X, RPS18, and RPS16. e HepG2 cells were treated with USP1 siRNAs or control siRNAs for 48 h. The expression levels of USP1, RPS4X, RPS18, and RPS16 were determined by western blot. GAPDH was used as a loading control. f HepG2 cells were treated with indicating doses of ML323 for 24 h. The expression levels of RPS4X, RPS18, and RPS16 were determined by western blot. g HepG2 cells were treated with bortezomib (BTZ, 50nM) for 24 h. Western blot was performed to determine the expression level of RPS16. h Linear structure models of wide type USP1 (USP1-WT) and its truncated mutants (USP1-TMs). i HEK293T cells were transfected with HA-RPS16 and FLAG-USP1-WT or FLAG-USP1-TMs. FLAG was immunoprecipitated from HEK293T cells and immunoblotted to HA and FLAG

Back to article page