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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: USP1-dependent RPS16 protein stability drives growth and metastasis of human hepatocellular carcinoma cells

Fig. 4

Inhibition of USP1 reduces proliferation of HCC cells. a HepG2, HCCLM3, Hep3B, and Huh7 cells were exposed to ML323 for 48 h. Cell viability was determined by MTS assay from three independent repeats. b The indicated HCC cells were treated with USP1 siRNAs or control siRNAs for 72 h. Cell viability was determined by MTS assay from three independent repeats. c The knockdown efficiency of USP1 was determined by western blot. d HepG2 and HCCLM3 cells were treated with ML323 or USP1 siRNAs for 48 h. DNA duplicate was determined by EdU staining assay. Representative images are shown. Scale bars, 50 μm. and f Relative intensities of the images were calculated and quantified from three independent repeats. g HepG2 and HCCLM3 cells were treated with ML323 for 48 h. Colony formation assay was performed for 2 weeks. h Colony formation rate was calculated and quantified from three independent repeats

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