Fig. 4

Inhibition of USP1 reduces proliferation of HCC cells. a HepG2, HCCLM3, Hep3B, and Huh7 cells were exposed to ML323 for 48 h. Cell viability was determined by MTS assay from three independent repeats. b The indicated HCC cells were treated with USP1 siRNAs or control siRNAs for 72 h. Cell viability was determined by MTS assay from three independent repeats. c The knockdown efficiency of USP1 was determined by western blot. d HepG2 and HCCLM3 cells were treated with ML323 or USP1 siRNAs for 48 h. DNA duplicate was determined by EdU staining assay. Representative images are shown. Scale bars, 50 μm. e and f Relative intensities of the images were calculated and quantified from three independent repeats. g HepG2 and HCCLM3 cells were treated with ML323 for 48 h. Colony formation assay was performed for 2 weeks. h Colony formation rate was calculated and quantified from three independent repeats