Fig. 6

USP1-promoted cell migration depends on RPS16 status. HepG2 and HCCLM3 cells were treated with RPS16 siRNAs or control siRNAs for 72 h. a Cell viability was determined by MTS assay. b DNA duplicate was determined by EdU staining assay. c and d Cell migration rate was determined by transwell migration assay. Representative images and quantitative data are shown. e Expressions of RPS16, Twist1 and Snail were determined by western blotting. f-i HepG2 and HCCLM3 cells were treated with ML323 in the absence or presence of HA-RPS16 for 72 h, or HepG2 and HCCLM3 cells stably expressing USP1 shRNAs or control shRNAs with or without the transfection of HA-RPS16 for 72 h. f Cell migration was determined by transwell assays. Representative images were shown. Scale bar, 50 μm. g Quantification of the percentage transwell/migrated cells was shown. h and i The protein levels of RPS16, Twist1, Snail, and HA were determined by western blotting analysis