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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Nuclear Transglutaminase 2 interacts with topoisomerase II⍺ to promote DNA damage repair in lung cancer cells

Fig. 2

Occurrence of DNA-PKcs dependent nuclear translocation of TG2 in response to DNA double strand breaks. A: Representative images for immunofluorescence staining of TG2 (green) in A549 cells at different times after exposure to 8 Gy γ-irradiation. Scale bar, 20 μm. B: Immunoblot of TG2 in both nucleus and cytoplasm lysates isolated from irradiated A549 cells. The levels of TG2 protein expressions in both Nuclear and cytoplasm were quantified with image J software. C: Localizations of TG2 in the same cells were determined in response to the DSB treatments of KU55933 (10µM), NU7441 (10µM) or VE821 (10µM). Scale bar, 20 μm. D: The ratio of nuclear TG2 and cytoplasm TG2 was quantified and analyzed. E: Representative images for immunofluorescence staining of TG2 in both DNA-PKcs proficient cells (M059K cells) and deficient cells (M059J cells) at 0.5 h after 8 Gy irradiation. F: Immunoprecipitations of TG2 were performed with TG2 antibody for the irradiated A549 cells, which was followed by the performed detection of p-SQ/TQ motif on TG2. G: The design of DNA-PKcs relative TG2 phosphorylation site mutant. H, I: wt-TG2-GFP, S68A-GFP, T162A-GFP and S68A + T162A-GFP expressing cells were treated with Etoposide (100 µg/ml, 0.5 h) and images were taken. Scale bar, 50 μm. Hoechst 33,343 was used as an indicator of nucleus. The ratio of nuclear TG2 and cytoplasm TG2 was quantified and analyzed. **P < 0.01

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