Fig. 3

AP-2α is a key transcription factor in POSTN mediated liver cancer cell CD133-positive transformation. a Oligonucleotide microarray analysis results of top 50 up-regulated genes in hep3B cell after transfected with control and p-POSTN plasmid; b Oligonucleotide microarray analysis results of top 50 down-regulated genes in HCCLM3 cell after transfected with lentiviral packaged shRNA targeting POSTN and matched scrambled RNA; c Venn diagram shows genes associated with (a), (b) combined with 19 transcriptional regulators of CD133 genes predicted by the TRANSFAC transcription factor prediction database. d-f FOXP3, c-Myc, AP-2α mRNA (d-e) and protein(f) level were compared in Hep3B cells transfected with control or p-POSTN plasmid as well as in HCCLM3 cells transfected with lentiviral packaged shRNA targeting POSTN or scrambled RNA. Data represent mean + SEM of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001. g The expression of POSTN mRNA was positively correlated with AP-2α mRNA using data from TCGA; h-i The expression of POSTN mRNA was negatively correlated with FOXP3 and c-Myc mRNA