Fig. 7
Co-expression of POSTN and AP-2α in liver cancer is an important indicator of poor prognosis. a Immunohistochemical staining was used to detect the expression of AP-2α in liver cancer tissue chip. The left side was highly differentiated liver cancer tissue, and the right side was poorly differentiated liver cancer tissue. The magnification of the image above was 50x, and the image below was 200x; b The staining of AP-2α protein in POSTNHigh and POSTNLow HCC tissues were compared by Chi square test; c Waterfall plot demonstrated that AP-2α overexpression were found in 68% of HCC cases; d Analysis of TCGA data revealed that HCC patients with high AP-2α expression (Z score > 1) were significantly associated with poorer overall survival; e Analysis of TCGA data revealed that HCC patients with high AP-2α expression (Z score > 1) were significantly associated with poorer disease-free survival; f Analysis of TCGA data revealed that the expression of AP-2α mRNA was positively correlated with the expression of TGFβ1 mRNA; g Using POSTN and AP-2α stratification analysis to confirm that HCC patients with high expression levels of both periostin and AP-2α had the worst prognosis; h Using POSTN and TGFβ1 stratification analysis to confirm that HCC patients with high expression levels of both POSTN and TGFβ1 had the worst prognosis compared to that of the Lower periostin/TGFβ1 group; i The flow chart of POSTN/TGFβ1 positive feedback pathway using AP-2α to promote the transformation and malignant process of liver cancer cells