Fig. 3
From: Metformin induces Ferroptosis by inhibiting UFMylation of SLC7A11 in breast cancer
Metformin causes System Xc− dysfunction. A MCF7 and T47D cells were treated with metformin at 0, 2, 5, or 10 mM for 48 h or treated with metformin (5 mM) for 0, 12, 24, 36, or 48 h. SLC7A11 and GPX4 protein expression was measured via Western blotting. B The relative SLC7A11 RNA level was measured via qRT-PCR after MCF7 and T47D cells were treated with metformin at 0, 2, 5, or 10 mM for 48 h. C T47D cells were treated with metformin (0–80 mM) for 48 h with or without the overexpression of SLC7A11. Cell viability was assayed. D T47D cells were treated with 5 mM metformin for 48 h with or without the overexpression of SLC7A11. The relative levels of GSH were assayed