Fig. 3

CARMN inhibits proliferation and sensitizes cisplatin in TNBC cells in vitro and in vivo. A Overexpression efficiency of CARMN confirmed by RT-qPCR. B Influence of CARMN overexpression on cell proliferation in TNBC cells detected by CCK8 assay. C Influence of CARMN overexpression on cell colony formation in TNBC cell. D Influence of CARMN overexpression on cell migration detected by transwell assays. E Effect of CARMN overexpression on sensitivity to cisplatin in TNBC cells reflected by relative activity of cells treated with different cisplatin concentration. F Cell apoptosis induced by cisplatin in TNBC cells overexpressing CARMN or control cells. G, H Xenograft tumors of 4 groups: CARMN overexpressed MDA-MB-468 cells + cisplatin; CARMN overexpressed MDA-MB-468 cells + saline; control MDA-MB-468 cells + cisplatin; control MDA-MB-468 + saline. G shows photos of xenograft tumors; H reflects volume and weight of xenograft tumors respectively. Scale bar = 1 cm. I HE staining and expression of Ki67 and PCNA in xenograft tumors with different expression of CARMN. Scale bar = 100 μm. J TUNEL assay of 4 groups of xenografts described above. Scale bar = 200 μm. IC50, 50% inhibition concentration; cDDP: cisplatin. Error bars represent means ± SD, *P < 0.05, **P < 0.01, ***P < 0.001