Fig. 5

CARMN overexpression causes suppressed DNA replication and altered cell cycle distribution in TNBC. A EdU foci in CARMN overexpressed cells and control ones treat with cisplatin (5 μg/ml) or blank. Scale bar = 100 μm. B MCM5 immunofluorescent staining in TNBC cells overexpressing CARMN and control cells treated with or without cisplatin (5 μg/ml). Scale bar = 200 μm. C Effect of CARMN overexpression on cell cycle distribution of TNBC cells. D Effect of cisplatin (5 μg/ml) on CARMN expression in TNBC cells with or without CARMN overexpression. cDDP: cisplatin. Error bars represent means ± SD, *P < 0.05, **P < 0.01, ns: no significance