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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Correction to: Pre-metastatic niche triggers SDF-1/CXCR4 axis and promotes organ colonisation by hepatocellular circulating tumour cells via downregulation of Prrx1

Fig. 3

Loss of Prrx1 is essential for SDF-1-induced stemness and migratory potential of HCC cells. a Migrating cells were counted under a microscope in five randomly selected fields. Bars represent number of migrated cells. b Drug resistance (epirubicin) was evaluated in indicated cells by CCK-8 assay. c Phase-contrast images of sphere-forming assays of indicated cells. d Western blotting and RT-qPCR were used to detect protein and mRNA expression of stemness markers, respectively. e Invading cells were counted under a microscope in five randomly selected fields. Bars represent number of migrated cells. f Drug resistance (epirubicin) was evaluated in indicated cells by CCK-8 assay. g Phase-contrast images of sphere-forming assays of indicated cells. h Western blotting was performed to detect the expression of stemness markers. * P < 0.05, ** P < 0.01

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