Fig. 6

Knockdown of miR-195-5p rescues the sh-circ_001422-mediated inhibitory effects on OS cell proliferation and metastasis. a & b. OS cells were transfected with sh-circ_001422-NC or sh-circ_001422 or were cotransfected with sh-circ_001422 and the miR-195-5p inhibitor. EdU incorporation (scale bar, 100 μm) and colony formation assays were performed to evaluate the proliferation ability of OS cells in each group. c & d. Flow cytometry was used to evaluate the effect of miR-195-5p silencing on the sh-circ_001422-mediated effects on the cell cycle distribution and apoptosis. e & f. Transwell migration and Matrigel invasion assays were performed to evaluate the effect of miR-195-5p silencing on the sh-circ_001422-mediated reductions in the migration and invasion abilities. Scale bar, 100 μm. g. The protein levels of CCND1, CDK4, cleaved CASP3, BAX, BCL2, E-cadherin, N-cadherin and Vimentin were analyzed by western blotting and normalized to the level of GAPDH. n.s., not significant; ^*P < 0.05; ^**P < 0.01; ^***P < 0.001