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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Circular RNA circ_001422 promotes the progression and metastasis of osteosarcoma via the miR-195-5p/FGF2/PI3K/Akt axis

Fig. 7

Circ_001422 positively regulates FGF2 expression in OS cells by sponging miR-195-5p. a. Cluster heatmap showing the significantly differentially expressed mRNAs between 143B cells transfected with sh-circ_001422 and 143B cells transfected with sh-circ_001422-NC. b. Venn diagram showing the overlapping target mRNAs of miR-195-5p predicted by TargetScan, miRanda, RNAhybrid and miRTarBase. c. The overlapping mRNAs between the downregulated mRNAs and miR-195-5p target mRNAs were subjected to KEGG enrichment analysis. d & e. qRT-PCR showed the relative quantification (RQ) values of 6 candidate genes in OS cells transfected with sh-circ_001422 or sh-circ_001422-NC. f & g. qRT-PCR showed the RQ values of 6 candidate genes in OS cells transfected with OE-circ_001422 or OE-circ_001422-NC. h & i. Western blot analysis showed the protein levels of FGF2, p-PI3K, total PI3K (t-PI3K), p-Akt and total Akt (t-Akt) in OS cells transfected with sh-circ_001422 or OE-circ_001422 or the corresponding negative control. j & k. Western blot analysis showed the protein levels of FGF2, p-PI3K, t-PI3K, p-Akt and t-Akt in OS cells transfected with the miR-195-5p mimic or miR-195-5p inhibitor or the corresponding negative control. l & m. Western blot analysis was performed to evaluate the effect of miR-195-5p silencing (or miR-195-5p overexpression) on sh-circ_001422 (or OE-circ_001422)-mediated changes in the protein levels of FGF2, p-PI3K, t-PI3K, p-Akt and t-Akt. n. The putative binding sites between miR-195-5p and FGF2. o. Luciferase activity was assessed in OS cells after cotransfection with FGF2-WT or FGF2-MUT and the miR-195-5p mimic or NC mimic. p. Representative images of immunohistochemical staining of FGF2 in OS tissues and paired noncancerous tissues. Scale bar, 100 μm. q. FGF2 protein expression in 12 OS tissues (T) and paired noncancerous tissues (N). n.s., not significant; *P < 0.05; **P < 0.01; ***P < 0.001

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