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Fig. 9 | Journal of Experimental & Clinical Cancer Research

Fig. 9

From: Circular RNA circ_001422 promotes the progression and metastasis of osteosarcoma via the miR-195-5p/FGF2/PI3K/Akt axis

Fig. 9

Gain-of-function experiments confirm the involvement of the circ_001422/miR-195-5p/FGF2 axis in OS progression and metastasis. a. Stable OE-circ_001422 OS cells were transfected with the miR-195-5p mimic (mim) or cotransfected with the miR-195-5p mimic and OE-FGF2. An EdU incorporation (scale bar, 100 μm) assay was used to evaluate the effect of circ_001422/miR-195-5p/FGF2 axis modulation on cell proliferation. b. Transwell migration and Matrigel invasion assays were performed to evaluate the effect of circ_001422/miR-195-5p/FGF2 axis modulation on the migration and invasion abilities of 143B cells. Scale bar, 100 μm. c. The protein levels of CCND1, CDK4, cleaved CASP3, BAX, BCL2, E-cadherin, N-cadherin, Vimentin, p-PI3K, t-PI3K, p-Akt and t-Akt were analyzed by western blotting and normalized to the level of GAPDH. d. Nude mice were distributed into 4 groups: control, OE-circ_001422, OE-circ_001422 + miR-195-5p agomir (ago), and OE-circ_001422 + miR-195-5p agomir + OE-FGF2. Representative images of xenograft tumors in the 4 groups are shown. e & f. Effect of circ_001422/miR-195-5p/FGF2 axis modulation on tumor growth and tumor weight in nude mice. g & h. Representative images of lung tissues and lung sections stained with H&E in the lung modulation mouse model. Scale bar, 1000 μm. i. Detection of Ki-67, PCNA, E-cadherin, N-cadherin and Vimentin expression in tumor samples from nude mice in the 4 groups by IHC. Scale bar, 100 μm. n.s., not significant; *P < 0.05; **P < 0.01; ***P < 0.001

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