Fig. 4

Circ_0084615 bound to miR-599 in CRC cells. (A) The targets of circ_0084615 were predicted by starbase and circinteractome. (B-E) The relationship between circ_0084615 and miR-599 was analyzed by RIP and RNA pull-down assays. (F) The complementary sequences between circ_0084615 and miR-599. (G) The expression of miR-599 in SW620 and DLD-1 cells transfected with miR-NC or miR-599 was detected by qRT-PCR assay. (H and I) The interaction between circ_0084615 and miR-599 was examined by dual-luciferase reporter assay. (J and K) The expression of miR-599 in CRC tissues and cells was determined by qRT-PCR assay. *P < 0.05, **P < 0.01, ***P < 0.001