Fig. 5
Inhibition of miR-599 rescued the impacts of circ_0084615 knockdown on CRC cell malignant behaviors. (A) The expression of miR-599 in SW620 and DLD-1 cells transfected with anti-NC or anti-miR-599 was detected by qRT-PCR assay. (B-H) SW620 and DLD-1 cells were transfected with sh-NC + anti-NC, sh-circ_0084615 + anti-NC or sh-circ_0084615 + anti-miR-599. (B-F) The proliferation, colony formation, migration, invasion and angiogenesis of SW620 and DLD-1 cells were evaluated via EdU assay, colony formation assay, wound-healing assay, transwell assay and tube formation assay, respectively. (G and H) The protein levels of ZEB2, E-cadherin, Vimentin and VEGFA in SW620 and DLD-1 cells were measured via western blot assay. *P < 0.05, **P < 0.01, ***P < 0.001