Fig. 1

GSCs cultured under hypoxia in endothelial conditions upregulate endothelial markers. Cytofluorimetric evaluation of CD31, CD34, Tie2, VEGFR2/KDR and CD133 endothelial marker expression after two and three weeks of culture in endothelial conditions under hypoxia. Four GSC lines (i.e. GSC#1, #61, #83 and #163) isolated from different GBM patients are shown. The baseline (dashed line at value 1) represents the expression of the same markers in the four GSC lines cultivated in stem cell medium in normoxia conditions, used as reference. Results are reported as mean ± SD (n = 3)