Fig. 3

Elesclomol induced a dose dependent increase of cell death and mitochondrial ROS. A Fluorimetric evaluation of viability of four different GSC lines grown in stem cell (GSCs, left panel) or in endothelial medium (GdECs, right panel) after treatment with 10, 100 and 1000 nM elesclomol and staining with calcein-AM (which is retained in the cytoplasm of live cells). Numbers represent calcein-positive cells, calculated as percentage of control untreated cells. Fluorimetric evaluation of MMP (by TMRM dye), cytoplasmic ROS (by DHR123 dye), mitochondrial ROS (by MitoSox red dye), and GSH (by MCB dye) in B GSCs and C GdECs. Results obtained from three independent experiments performed in triplicate are reported as means ± SD