Fig. 4

Reverse-Phase Protein microArrays (RPPA) analysis of HMVECs, GSCs and GdECs challenged with elesclomol. A NMF mixture coefficient and consensus map matrices heatmaps. The main clusters of samples correspond to individual cell lines, irrespective of growth conditions as well as of elesclomol concentration and time point. Color value ranges are reported at top-right of individual heatmaps. Color legends for annotation bars in both heatmaps are reported at the right of the panel. B PCA plot of dimensions 1 and 2. Sample and concentration scale legends are reported on the right of the plot. C Venn diagram of genes corresponding to significant antibodies emerging from statistical comparisons of vehicle and elesclomol samples, in GSCs grown in stem cell (GSCs) or endothelial cell (GdECs) conditions. The list of genes for each group (GSCs, GdECs, common) is available as Supplementary Table 4. D Histograms of the enrichment ratio of KEGG pathways relative to GSC- or GdEC-unique genes. Images were produced using the WEB-based GEne SeT AnaLysis Toolkit (WebGestalt, http://www.webgestalt.org/). E Representative PI3K-Akt Signaling and Focal Adhesion KEGG pathways showing mapped GdEC-unique genes and inferred network paths. Images were produced using the KEGG Mapper tool (Search Pathway, https://www.genome.jp/kegg/mapper.html)