Fig. 4

Expression of cancer-intrinsic immune-related genes. (A) Expression of immune- and immune checkpoint-related genes by CCOs and primary tissues. (B) Variable expression of immune-related genes by CCOs, specifically HLA class II genes. (C) CD274 (PDL1) expression in CCOs and primary tumor tissues. There was no correlation in CD274 expression between them. (D) CD274 expression based on the molecular subgroups with higher expression in the k4 (mesenchymal) subgroup (Kruskal-Wallis test). (E) Frequencies of mutated genes based on the CD274 expression level of the CCOs. (F) Enriched signaling pathways using GO (gene ontology) term database in CCOs with higher CD274 expression. (G) Enriched signaling pathways using hallmark gene set database in CCOs based on CD274 expression levels. CCO, colorectal cancer organoid